mouse anti-aurora kinase antibody (Merck & Co)
90
Structured Review
Merck & Co
mouse anti-aurora kinase antibody
Mouse Anti Aurora Kinase Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-aurora+kinase+antibody/a1231/bio_rxiv__2023__08__08__552464-257-15-22
Average 90 stars, based on 1 article reviews
Mouse Anti Aurora Kinase Antibody, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-aurora+kinase+antibody/a1231/bio_rxiv__2023__08__08__552464-257-15-22
Average 90 stars, based on 1 article reviews
mouse anti-aurora kinase antibody - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Spatially distinct inputs modulate the amount of active Mitotic-phase GAP to locally restrict RhoA signaling for successful cell division"
Article Title: Spatially distinct inputs modulate the amount of active Mitotic-phase GAP to locally restrict RhoA signaling for successful cell division
Journal: bioRxiv
doi: 10.1101/2023.08.08.552464
Figure Legend Snippet: A) Aurora A kinase could facilitate RhoA inactivation by activating the RhoA GAP MP-GAP. B) Schematic of MP-GAP domain organization highlighting the three fragments and putative phosphosites tested in the kinase assays. C) Coomassie gel and autoradiography images of Aurora A kinase assays with GST-tagged GAP domain and F1 and F2 region of MP-GAP. Different lanes of the same gel are shown. D) Sequence alignment of the F1 region of MP-GAP. The conserved putative Aurora A phosphosites are highlighted in red. E, F) Coomassie gel and autoradiography images of Aurora A kinase assays with the myelin basic protein (MBP, positive control), GST (negative control), GST-tagged F1-WT, F1-C1 (S285A, S304A, S345A) and F1-C2 (S403A, S434A, S500A, T508A) (E) or F1-S285A, F1-S304A, and F1-S345A (F) of MP-GAP.
Techniques Used: Autoradiography, Sequencing, Positive Control, Negative Control
Figure Legend Snippet: A) Merged immunofluorescent images showing PLA foci (white) and DNA (red) for metaphase and anaphase HeLa cell. Staining was performed either with the anti-Aurora A and anti-MP-GAP together (positive) or only with one antibody (negative). B) Distribution of the PLA foci in % at the cell periphery, middle or center during metaphase and anaphase. C) The number of PLA foci per 100 µm 2 is plotted for the central or polar area of the cell. D) Immunofluorescence images of F-actin (white) and DNA (red) stained HeLa cells treated with DMSO or 2.5 µM Latrunculin A (LatA). E) Immunofluorescence images of MP-GAP (white) and DNA (red) stained HeLa cells treated with DMSO or 2.5 µM Latrunculin A. F) MP-GAP fluorescence intensity is plotted for an equatorial linescan. Examples of an equatorial and polar linescan are shown for illustration. G) Quantification of the mean cortical MP-GAP intensity as depicted in (F) for the entire cell cortex in metaphase and for the poles and equator in anaphase cells for indicated treatments. Values above the dotted line represent cortical MP-GAP enrichment. H) Shown are immunofluorescence images of metaphase and anaphase HeLa cells treated with DMSO or MK-5108. Cells were stained for MP-GAP (white) and DNA (red). I) Shown are immunofluorescence images of metaphase and anaphase HeLa cells expressing GFP-MP-GAP WT or GFP-MP-GAP S3A and treated with MP-GAP siRNA. Cells were stained for MP-GAP (white) and DNA (red). J) Quantification of the mean cortical MP-GAP intensity as depicted in (F) for the entire cell cortex in metaphase and for the poles and equator in anaphase cells for indicated treatments. For all n = number of cells, and ≥2 independent experiments were performed for each condition. Error bars are SEM, and p values were calculated with the Kruskal-Wallis test. Scale bars are 5 µm.
Techniques Used: Staining, Immunofluorescence, Fluorescence, Expressing
Figure Legend Snippet: A) Scheme of the possible autoinhibitory conformation of MP-GAP (left). Coomassie gel and anti-His probed immunoblot of pull down assays incubating immobilized GST-tagged MP-GAP fragments (F1, F2 and GAP) with soluble 6xHis-tagged GAP domain (right). B) Scheme of the putative position of the Aurora A phosphorylation sites in either the hinge region (left) or on the binding surface (right) of F1. Coomassie gel and anti-His probed immunoblot of pull down assays incubating immobilized GST-tagged F1 wild type or F1 S3D with soluble 6xHis-tagged GAP domain (right).
Techniques Used: Western Blot, Binding Assay
Figure Legend Snippet: A) In normal cells Aurora A signal is high at the poles and Ect2 autoinhibition is locally released at the cell equator (top). Abolishing Ect2 autoinhibition by the W307A mutation, results in active Ect2 throughout the cell (middle). Without Aurora A signaling, Ect2 autoinhibition is still locally released at the equator (bottom). In all conditions cytokinesis succeeds. B) Merged time-lapse transmission and DNA (SiR-DNA, red) images of HeLa cells expressing Ect2 WT or Ect2 W307A . Cells were treated during live-cell imaging around anaphase onset with DMSO or MK-5108 as indicated. C, D) Percentage of cytokinesis failure (C) and equatorial cell width (D) of the conditions filmed in (B). E) Two spatially distinct regulatory inputs control MP-GAP function at the cell equator and the cell poles during anaphase. At the cell poles F-actin levels are low, but Aurora A kinase activity is high. Aurora A phosphorylates the hinge region between the GAP domain and the F1 region resulting in opening and activation of MP-GAP and RhoA inactivation. At the cell equator Ect2 autoinhibition is released, RhoA is activated and promotes F-actin polymerization. Due to high F-actin levels MP-GAP enriches at the equator and increases the GTP hydrolysis rate of RhoA resulting in high RhoA flux and narrowing of the active RhoA zone. For all n = number of cells, and ≥2 independent experiments were performed for each condition. Scale bars are 5 µm.
Techniques Used: Mutagenesis, Transmission Assay, Expressing, Live Cell Imaging, Activity Assay, Activation Assay
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